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NEXTflex Small RNA Sequencing Kit for NGS Library Prep Launched - Bioo Scientific’s new patent pending NEXTflex™ Small RNA Sequencing Kit offers an easy, flexible, cost-effective solution for generating libraries from small RNA - BiooScientific.com
NEXTflex Small RNA Sequencing Kit for NGS Library Prep Launched

 

PRZOOM - /newswire/ - Austin, TX, United States, 2011/05/04 - Bioo Scientific’s new patent pending NEXTflex™ Small RNA Sequencing Kit offers an easy, flexible, cost-effective solution for generating libraries from small RNA - BiooScientific.com.

   
 
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Bioo Scientific launched the patent pending NEXTflex™ Small RNA Sequencing Kit which provides an easy, flexible, cost-effective solution for generating single and multiplexed libraries from small RNA using Illumina GAIIx and HiSeq 2000 sequencing platforms. The NEXTflex Small RNA Sequencing Kit allows users to generate libraries directly from as little as 1 μg of total RNA. This kit contains a specialized 3’ adenylated adapter that specifically ligates onto microRNAs and other small RNAs containing a 3’ hydroxyl group. After adapter ligation and reverse transcription, libraries are amplified using a barcoded primer, allowing up to 48 samples to be multiplexed and sequence variations interrogated. The NEXTflex Small RNA Sequencing Kit incorporates AIR™ Ligase, a patent pending, truncated T4 RNA Ligase, which significantly increases the efficiency with which small RNAs are tagged with adapter, giving greater sequence depth. The new protocol is designed to significantly reduce adapter dimer formation prevalent in other protocols.

Strand specificity is retained through the use of adapters that ligate to the 5’‐phosphate and 3’‐hydroxyl groups that most mature miRNAs possess. The kit can be used for mRNA directional sequencing. In combination with the NEXTflex™ Small RNA Barcode Primer kits, 48 samples can be profiled and studied simultaneously. Four sets of 12 NEXTflex™ Small RNA Barcode Primers, with embedded index sequences are available offering an improved multiplexing workflow and increased flexibility. This new automation-friendly format enables multiplexing of up to 384 samples. The ability to pool samples in an efficient way significantly decreases hands on time while providing robust data quality. The primer barcoding system utilizes a 6 nt index to differentiate up to 48 different samples on a single flow cell lane. In addition to small RNA sequencing, the NEXTflex Small RNA Sequencing Kit is compatible with mRNA directional, RIP-Seq and CLIP-Seq protocols.

The NEXTflex Small RNA Sequencing Kit and NEXTflex Small RNA Barcode Primers are the latest additions to Bioo Scientific’s portfolio of next generation sequencing solutions. These kits demonstrate Bioo Scientific’s commitment to developing innovative solutions to improve, simplify and reduce the costs of next generation sequencing.

About Bioo Scientific Corporation
Bioo Scientific Corporation (biooscientific.com) is an Austin, TX based biotechnology company that provides innovative solutions to the life science industry. Bioo Scientific offers a complete portfolio of products that increase sensitivity, flexibility and speed to next-generation sequencing.

Bioo Scientific’s products are used around the world and can be purchased both directly or through our global distribution network with markets in more than 40 countries. For more information about Bioo Scientific’s NGS product line, visit the website.

 
 
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NEXTflex Small RNA Sequencing Kit for NGS Library Prep Launched

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Contact: Dawn Obermoeller - BiooScientific.com 
512-707-8993 dawno[.]biooscientific.com
 
PRZOOM / PRTODAY - Newswire Today disclaims any content contained in this article. If you need/wish to contact the company who published the current release, you will need to contact them - NOT us. Issuers of articles are solely responsible for the accuracy of their content. Our complete disclaimer appears here.
IMPORTANT INFORMATION: Issuance, publication or distribution of this press release in certain jurisdictions could be subject to restrictions. The recipient of this press release is responsible for using this press release and the information herein in accordance with the applicable rules and regulations in the particular jurisdiction. This press release does not constitute an offer or an offering to acquire or subscribe for any Bioo Scientific securities in any jurisdiction including any other companies listed or named in this release.

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