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AnaSpec Introduces Industry’s First FRET-Based – SensoLyte® Green Elastase Assay Kits - AnaSpec, Eurogentec Group is pleased to introduce the SensoLyte® Green Elastase Assay Kit - the first FRET-based Elastase activity assay kit in the industry
AnaSpec Introduces Industry’s First FRET-Based – SensoLyte® Green Elastase Assay Kits

 

PRZOOM - /newswire/ - Fremont, CA, United States, 2011/02/02 - AnaSpec, Eurogentec Group is pleased to introduce the SensoLyte® Green Elastase Assay Kit - the first FRET-based Elastase activity assay kit in the industry.

   
 
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This kit employs a natural substrate, elastin that is labeled with the 5-FAM fluorophore and the QXL™ 520 quencher. Proteolytic cleavage of labeled elastin separates the donor from the quencher and yields brightly green fluorescence, which can be continuously monitored at excitation/emission (Ex/Em) = 488 nm/520 nm. Increase in fluorescence intensity is directly proportional to elastase activity. The kit can be used to continuously measure the kinetics of elastase activity. As well, this highly sensitive kit is homogeneous – it does not require any separation steps.

Elastases catalyze the hydrolysis of elastin, the chief constituent of elastic fibers that together with collagen determines the mechanical properties of connective tissue. Elastases have many other natural substrates these include proteoglycans, collagens and fibronectin.1-4 Implicated in many diseases such as pulmonary emphysema, cystic fibrosis, infections, inflammation and atherosclerosis, elastases are considered important drug targets.5

AnaSpec, Eurogentec Group also provides another green emitting elastase assay, the SensoLyte® Rh110 Elastase Assay Kit. This assay uses a substrate that is labeled with a fluorogenic dye called Rh110 (rhodamine 110). Upon cleavage by elastase, the substrate generates free Rh110 fluorophore, which has a bright green fluorescence. Signal can be detected at Ex/Em =490/520 nm. The longer wavelength spectra and higher extinction coefficient of this kit and the SensoLyte® Green Elastase kits provide greater sensitivity and less interference from other reaction components.

References
1. Kafienah, W. et al. Biochem J. 330, 897 (1998).
2. Carlo, L. et al. J. Biol. Chem. 255, 12006 (1980).
3. Mainardi, CL. et al. J. Biol. Chem. 255, 5435 (1980).
4. McDonald, JA. et al. J. Biol. Chem. 255, 8848 (1980).
5. Bieth, JG. J. Soc. Biol. 195, 173 (2001).

About AnaSpec, Eurogentec Group
AnaSpec (anaspec.com), Eurogentec Group is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. As a subsidiary of Eurogentec, AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, oligonucleotides, and qPCRs. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery. AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development and oligonucleotide synthesis. AnaSpec is certified to ISO 9001:2008.

 
 
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AnaSpec Introduces Industry’s First FRET-Based – SensoLyte® Green Elastase Assay Kits

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Contact: Tiffany Kao - AnaSpec.com 
510-791-9560
 
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IMPORTANT INFORMATION: Issuance, publication or distribution of this press release in certain jurisdictions could be subject to restrictions. The recipient of this press release is responsible for using this press release and the information herein in accordance with the applicable rules and regulations in the particular jurisdiction. This press release does not constitute an offer or an offering to acquire or subscribe for any AnaSpec, Inc. securities in any jurisdiction including any other companies listed or named in this release.

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