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AnaSpec Introduces Beta - Secretase Enzyme & Assay - As one of the world’s largest providers of beta-amyloid peptides and Alzheimer’s related research products, AnaSpec has announced its latest offering – the human recombinant beta-secretase enzyme
AnaSpec Introduces Beta - Secretase Enzyme & Assay

 

PRZOOM - /newswire/ - Fremont, CA, United States, 2009/10/16 - As one of the world’s largest providers of beta-amyloid peptides and Alzheimer’s related research products, AnaSpec has announced its latest offering – the human recombinant beta-secretase enzyme.

   
 
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b- secretase (BACE1) is a key protease involved in the production of beta-amyloid peptides found in extracellular amyloid plaques of patients with Alzheimer’s disease (AD).1 This enzyme has been implicated as an important target for anti-amyloid therapy of AD.2

The recombinant human BACE1 contains the catalytic domain and a C-terminal His tag. The protein was expressed as inclusion bodies in E. coli. It was refolded and purified using ion exchange and IMAC chromatography.3 Enzyme activity can been measured in fluorescence resonance energy transfer (FRET) substrate based assays (e.g. SensoLyte® 520 beta-Secretase Assay Kit, cat# 71144). 100 ng of b-secretase is sufficient for this FRET-based assay.

AnaSpec’s SensoLyte™ 520 b-Secretase Assay Kit (cat# 71144) is the industry’s only long wavelength assay kit in the green range. This kit is a fluorimetric assay kit and is sensitive, robust and easy-to-use. It employs a FRET peptide (mentioned above), facilitating high throughput screening of beta-secretase targeting drug candidates. The sequence of this FRET peptide is derived from the beta-secretase cleavage site of beta-amyloid precursor protein (APP) with Swedish mutation. This mutation enhances the susceptibility of APP to beta-secretase and results in an early onset of AD.

References:

1. Willem, M. et al. Semin Cell Dev Biol. 20,175 (2009).
2. Ghosh, K. et al. Neurotherapeutics 5, 399 (2008).
3. Tomasselli, G. et al. Protein & Peptide Letters. 15,131 (2008).

About AnaSpec, Inc.

AnaSpec (anaspec.com) is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and antibodies.

 
 
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AnaSpec Introduces Beta - Secretase Enzyme & Assay

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Contact: Ping Yang - AnaSpec.com 
510-791-9560 ping[.]anaspec.com
 
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IMPORTANT INFORMATION: Issuance, publication or distribution of this press release in certain jurisdictions could be subject to restrictions. The recipient of this press release is responsible for using this press release and the information herein in accordance with the applicable rules and regulations in the particular jurisdiction. This press release does not constitute an offer or an offering to acquire or subscribe for any AnaSpec, Inc. securities in any jurisdiction including any other companies listed or named in this release.

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