Free press releases distribution network?

Agency / Source: The Scott Partnership

Check Ads Availability|e-mail Article


Are you the owner of this article?, Turn it PREMIUM with your LOGO instead - and make it 3rd party Ads-Free! within the next hour!



New Protein Folding Application Note Uses Wyatt Technology's Dynamic Light Scattering Technique - Wyatt Technology, with a new application note demonstrating the capability of its DynaPro dynamic light scattering instrument to monitor the dissociation of an oligomeric protein and unfolding of monomers simultaneously using chaotropic agents
New Protein Folding Application Note Uses Wyatt Technology's Dynamic Light Scattering Technique

 

PRZOOM - /newswire/ - Santa Barbara, CA, United States, 2008/09/15 - Wyatt Technology, with a new application note demonstrating the capability of its DynaPro dynamic light scattering instrument to monitor the dissociation of an oligomeric protein and unfolding of monomers simultaneously using chaotropic agents.

   
 
Your Banner Ad Here instead - Showing along with ALL Articles covering Pharma/BioTech/Nutrition Announcements

Replace these Affiliate Programs at ANYTIME! Your banner here within the next hour. Learn How!


 

Wyatt Technology, the world leader in absolute macromolecular characterization products, announces the publication of a new free-of-charge application note demonstrating the capability of its DynaPro dynamic light scattering instrument to monitor the dissociation of an oligomeric protein and unfolding of monomers simultaneously using chaotropic agents. The analysis of proteins is particularly important for the study of many diseases such as cancer, diabetes, cardiovascular disease and neurodegenerative diseases such as Alzheimer's.

The importance of protein folding and unfolding has long been recognized and is an active field of research. The main rules of protein folding/unfolding have been established from in vitro experiments and the information obtained can be related to the function and behavior of proteins in vivo. Unfolding of proteins in vitro can be induced chemically using chaotropic agents like guanidine hydrochloride or urea. Protein Y is a 16 kDa, almost fully helical human protein. At pH 5.0 it forms oligomers of different sizes. Studying the unfolding of protein Y by circular dichroism, the main transition in the secondary structure can be observed. The aim of this specific study was to follow the unfolding process by another independent method.

For this application, far UV CD spectra were taken at 25°C on an AVIV spectrophotometer using a 0.01 cm cell. 5 spectra were recorded with a step size of 1.0 nm, a bandwidth of 1.5 nm and an averaging time of 5 seconds.
Samples were prepared 3 hours before measurement to allow time for the unfolding. Protein concentration was 2 mg/ml. The inflexion point of the unfolding curve is at 2.6 M GnHCl by both methods. Dynamic light scattering was performed with a DynaPro using a 12?l quartz cuvette. Samples were filtered with a 0.01?m Whatman Anodisc-13 filter.

Data were analyzed with the easy-to-use Wyatt Dynamics software and 25-30 acquisitions were averaged. The main transition in secondary structure can be observed by estimating the Stokes radius of the protein under different concentrations of the chaotropic agent (Fig. 1.). The mid-point of the transition agrees exceptionally well with the CD data (2.6M GnHCl). However, applying DLS measurements it is possible to observe additional molecular events: the dissociation of oligomers in the 0-2 M GnHCl concentration range.

The results of the experiment clearly demonstrate that dynamic light scattering measurements are capable of quickly and easily monitoring the dissociation of an oligomeric protein and unfolding of monomers simultaneously using chaotropic agents.

About Wyatt
Based in Santa Barbara, California, Wyatt Technology is the world's leading provider of instruments for absolute macromolecular characterization. With over 35 years' experience developing multi-angle light scattering detectors, working with customers in the biotechnology, chemical, petrochemical, pharmaceutical, academic and government arenas, Wyatt prides itself on its entrepreneurial spirit, and the uniqueness of its offerings. The Company's groundbreaking technology and uncompromising levels of customer care make Wyatt the global hallmark in its field.

For further press information please contact: Laura Browne, The Scott Partnership, 1 Whiteside, Station Road, Holmes Chapel, Cheshire. CW4 8AA United Kingdom T: +44 1477 539539 F: +44 1477 539540 E: wyatt[.]scottpr.com.

 
 
Your Banner Ad Here instead - Showing along with ALL Articles covering Pharma/BioTech/Nutrition Announcements

Replace these Affiliate Programs at ANYTIME! Your banner here within the next hour. Learn How!


 

Agency / Source: The Scott Partnership

 
 

Availability: All Regions (Including Int'l)

 

Traffic Booster: [/] Quick PRZOOM - Press & Newswire Visibility Checker

 

Distribution / Indexing: [+]

 
 
# # #
 

 
  Your Banner Ad showing on ALL
Pharma/BioTech/Nutrition articles,
CATCH Visitors via Your Competitors Announcements!


New Protein Folding Application Note Uses Wyatt Technology's Dynamic Light Scattering Technique

Company website links NOT available to basic submissions
It is OK to republish and/or LINK any newswire for any legitimate media purpose as long as you name PRZOOM - Press & Newswire and LINK as the source.
 
  For more information, please visit:
Is this your article? Activate ALL web links by Upgrading to Press Release PREMIUM Plan Now!
|
Contact: Laura Browne 
+ 44 1477 539539 info[.]wyatt.com
 
PRZOOM / PRTODAY - Newswire Today disclaims any content contained in this article. If you need/wish to contact the company who published the current release, you will need to contact them - NOT us. Issuers of articles are solely responsible for the accuracy of their content. Our complete disclaimer appears here.
IMPORTANT INFORMATION: Issuance, publication or distribution of this press release in certain jurisdictions could be subject to restrictions. The recipient of this press release is responsible for using this press release and the information herein in accordance with the applicable rules and regulations in the particular jurisdiction. This press release does not constitute an offer or an offering to acquire or subscribe for any The Scott Partnership securities in any jurisdiction including any other companies listed or named in this release.

Pharma/BioTech/Nutrition via RSSAdd NewswireToday - PRZOOM Headline News to FeedBurner
Find who RetweetFollow @NewswireTODAY



Are you the owner of this article?, Turn it PREMIUM with your LOGO instead - and make it 3rd party Ads-Free! within the next hour!




Read Latest Press Releases From The Scott Partnership / Company Profile


Read Pharma/BioTech/Nutrition Most Recent Related Press Releases:

Frost & Sullivan Commends Nemaura for Developing Superior Transdermal Drug Delivery Technology Platforms, the Memspatch and Micropatch
Greenphire Sponsors Second Annual Clinical Trial Agreements, Investigator Payments and Fair Market Value Meeting
Genentech’s Gazyva Helped People with Previously Untreated Follicular Lymphoma Live Significantly Longer
BD Announces Adoption of Windows 10 ‘Internet of Things’ for the Pyxis™ ES System
Greenphire Named to Deloitte 2016 Technology Fast 500™
Genentech Showcases New Clinical Data Across a Variety of Blood Diseases at American Society of Hematology 2016 Annual Meeting
BD Collaborates with JDRF to Focus on Extended Wear Innovations for Insulin Infusion Delivery
BD Launches First Syringe Designed for Use with Humulin® R U-500 Insulin
MDxHealth Announces Unique CPT Code Awarded for ConfirmMDx from the American Medical Association
BD MAX™ Vaginal Panel Receives FDA Market Authorization to Detect Most Common Causes of Vaginal Infections

Boost Your Social Network
& Crowdfunding Campaigns


LIFETIME SOCIAL MEDIA WALL
 
NewswireToday Celebrates 10 Years in Business
Find business coaching, life coaching, executive coaching and corporate coaching, best selling coaching books, ...



PREMIUM Members


Visit  Triggr & Bloom

Visit  BizJobs.com

Visit  JobsWare.com







 
  ©2016 PRZOOM — Limelon Advertising, Co.
Home | About PRZOOM | Advertise/Pricing | Contact | Investors | Privacy/TOS | Sitemap | FRANCAIS
newswire, PR free press releases distribution service magazines engine news alert newsroom press room breaking news public relations articles company news alerts newswiredistribution ezine bizentrepreneur biznewstoday digital business report market search pr firms agencies reports distri-bution today investor relation successful internet entrepreneur newswire distribution prtoday.com freenewswiredistribution asianewstoday bizwiretoday USA pr UK today