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AnaSpec, Inc. Introduces New SensoLyte™ Cathepsin D Activity Assay Kits - AnaSpec has introduced a new series of SensoLyte™ Cathepsin D Activity Assay Kits to address the issue of autofluorescence interference
AnaSpec, Inc. Introduces New SensoLyte™ Cathepsin D Activity Assay Kits

 

PRZOOM - /newswire/ - San Jose, CA, United States, 2008/03/07 - AnaSpec has introduced a new series of SensoLyte™ Cathepsin D Activity Assay Kits to address the issue of autofluorescence interference.

   
 
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While the detection of cathepsin activity plays a crucial role in the research of a number of pathological processes, the sensitivity of traditional cathepsin assay kits has been significantly hampered by autofluorescence interference at lower wavelengths. AnaSpec has introduced a new series of SensoLyte™ Cathepsin D Activity Assay Kits to address the issue of autofluorescence interference.

The SensoLyte™ 520 Cathepsin D Activity Assay Kit offers Cathepsin D detection at the industry’s longest wavelength. Leveraging AnaSpec’s proprietary fluorescence technology, the SensoLyte™ 520 Cathepsin D Activity Assay Kit incorporates a FRET substrate that fluoresces at a much higher wavelength than standard substrates. The higher wavelength fluorescence results in cathepsin detection that avoids autofluorescence interference from cell components and test compounds.

The FRET substrate in the SensoLyte™ 520 Cathepsin D Activity Assay Kit utilizes a unique 5-FAM/QXL™520 FRET pair (Ex/Em=490/520 nm upon cleavage). The sequence of the FRET substrate is derived from the cleavage site of Cathepsin D. In the intact FRET peptides the fluorescence of donors is quenched by acceptors. When active Cathepsin D cleaves FRET substrate into two separate fragments, the fluorescence of the donor is recovered, and can be monitored.

AnaSpec also offers the SensoLyte™ 390 Cathepsin D Activity Assay Kit that uses a traditional Mc-Ala/Dnp FRET peptide for measurement of enzyme activity (Ex/Em=330/390 nm upon cleavage)

Cathepsin is a protease that breaks apart other proteins found in many types of cells. There are at least 12 members of the cathepsin family which are distinguished by their structure and the proteins that they cleave. Cathepsin D is the lysosomal aspartic proteinase implicated in intracellular protein degradation.

About AnaSpec, Inc.
AnaSpec, Inc. (anaspec.com) is a leading provider of integrated proteomics solutions to pharmaceutical, biotech, and academic research institutions throughout the world. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and combinatorial chemistry.

References:
1. Berchem, G. et al, Oncogene 21, 5951(2002)
2. Liaudet-Coopman, E. et al, Cancer Lett. 237, 167 (2006)
3. Davidson, Y. et al, J Neurol Neurosurg Psychiat.77, 515 (2006)
4. Laurent-Matha, V. et al, J Cell Biol. 168,489 (2005)
5. Simon, DI. et al, Biochemistry 33, 6555 (1994)
6. Baechle, D. et al, J Peptide Sci. 11, 166 (2005)
7. Yasuda, Y. et al, J Biochem. 125, 1137 (1999).

 
 
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AnaSpec, Inc. Introduces New SensoLyte™ Cathepsin D Activity Assay Kits

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Contact: Ping Yang 
408-452-5055 ping[.]anaspec.com
 
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IMPORTANT INFORMATION: Issuance, publication or distribution of this press release in certain jurisdictions could be subject to restrictions. The recipient of this press release is responsible for using this press release and the information herein in accordance with the applicable rules and regulations in the particular jurisdiction. This press release does not constitute an offer or an offering to acquire or subscribe for any AnaSpec, Inc. securities in any jurisdiction including any other companies listed or named in this release.

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