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Trevigen Launches High-Throughput PARP/Apoptosis Assays - Trevigen launches High-Throughput PARP/Apoptosis Assays ideal for measuring the activity of PARP in 96-well plates
Trevigen Launches High-Throughput PARP/Apoptosis Assays

 

PRZOOM - /newswire/ - Arlington, VA, United States, 2008/02/22 - Trevigen launches High-Throughput PARP/Apoptosis Assays ideal for measuring the activity of PARP in 96-well plates.

   
 
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Cell extracts can be obtained using our newly-developed, higher-throughput procedure before, during and after apoptosis induction.

During apoptosis, PARP-1 which catalyzes the NAD dependent addition of poly (ADP-ribose) (PAR) onto various cytoplasmic and nuclear proteins, is cleaved from about 116 kDa to 85 kDa. Trevigen's HT PARP/Apoptosis Assay is ideal for measuring the activity of PARP in cell extracts before and during apoptosis. The HT PARP/Apoptosis Assay is an ELISA which semi-quantitatively detects PAR deposited onto immobilized histone proteins in a 96-well format. An anti-PAR monoclonal antibody, goat anti-mouse IgG-HRP conjugate, and HRP substrate are used to generate a colorimetric signal. Thus, absorbance correlates with PARP activity. Etoposide is a topoisomerase II inhibitor that stabilizes this enzyme after it cleaves DNA. It is included as a control apoptosis inducer.

Trevigen offers two formats of the HT PARP/Apoptosis Assay: Cat#4684-096-K (Colorimetric) and Cat# 4685-096-K (Chemiluminescent). Both assays feature non-radioactive formats and high-throughput 96 test size, as well as sensitivity down to 0.1 mUnits of PARP -- equivalent to less than 500 cells/well. Additional histone-coated strip wells (Cat# 4677-096-P) are available separately. For more details about this product, contact Trevigen by phone at 800-873-8443, email or visit the website.

Trevigen, Inc.
8405 Helgerman Ct.
Gaithersburg, MD 20877
P: 1-800-873-8443 / F: 1-301-560-4973

 
 
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Agency / Source: Trevigen, Inc.

 
 

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Trevigen Launches High-Throughput PARP/Apoptosis Assays

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HT PARP Assay |
Contact: Belinda De Leon - Trevigen.com 
1-800-TREVIGEN info[.]trevigen.com
 
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IMPORTANT INFORMATION: Issuance, publication or distribution of this press release in certain jurisdictions could be subject to restrictions. The recipient of this press release is responsible for using this press release and the information herein in accordance with the applicable rules and regulations in the particular jurisdiction. This press release does not constitute an offer or an offering to acquire or subscribe for any Trevigen, Inc. securities in any jurisdiction including any other companies listed or named in this release.

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