AnaSpec has introduced a suite of fluorescently labeled GST and 6X-His antibodies. These antibodies are labeled with a selection of dyes, including AnaSpec’s proprietary HiLyte Fluor™ and HiLytePlus™ dyes, which span the visible spectrum. This gives researchers a wide range of wavelength choices for use in immunohistochemistry or immunofluorescence applications. Non-labeled or biotin labeled versions are also available.
Different gene expression systems have been used to produce recombinant proteins.1-2 Protein tags are peptide sequences genetically introduced onto a recombinant protein. Tags include short peptides(FLAG, c-myc, 6X-His) or proteins (glutathione-s-transferase - GST). This tagging technique aids in the characterization, localization or purification of the recombinant expressed protein.3 Antibodies raised against fusion-tags are useful in biochemical or immunohistochemistry applications.
About AnaSpec, Inc.
AnaSpec, Inc. is a leading provider of integrated proteomics solutions to pharmaceutical, biotech, and academic research institutions throughout the world. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and combinatorial chemistry.
1. Zheng et al. (2003) J. Biochem. 133: 577-581.
2. Amrein et al. (1995) PNAS. 92:1048-1052.
3. Nakajima K. and Yaoita Y. (1997) Nucleic Acids Res. 25: 2231-2232.
4. Medina et al. (2000) Nucleic Acids Res. 28: i-vi.