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AnaSpec Introduces New SensoLyte™ Beta-Secretase Assay Kit - Leveraging one of the world’s largest collections of amyloid peptides, AnaSpec has introduced its latest development in novel solutions for amyloid-related research - the new SensoLyte™ 520 Beta-Secretase Assay Kit
AnaSpec Introduces New SensoLyte™ Beta-Secretase Assay Kit

 

PRZOOM - /newswire/ - San Jose, CA, United States, 2007/06/20 - Leveraging one of the world’s largest collections of amyloid peptides, AnaSpec has introduced its latest development in novel solutions for amyloid-related research - the new SensoLyte™ 520 Beta-Secretase Assay Kit.

   
 
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Beta-secretase catalyzes a key step in the production of beta-amyloid peptides seen accumulated in senile plagues of Alzheimer’s disease (AD) brains. In order to facilitate high throughput screening of AD drug candidates, we have developed a new SensoLyte™ 520 beta-secretase assay kit using a fluorescence resonance energy transfer (FRET) peptide, HiLyte Fluor™ 488-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(QXL™ 520)-OH (see Figure 1). The sequence of this FRET peptide is derived from the beta-secretase cleavage site of beta-amyloid precursor protein(APP) with Swedish mutation.6 This mutation enhances the susceptibility of APP to beta-secretase and results in an early onset of AD.

This assay has good sensitivity (0.03 mU/ml) with a signal-to-background ratio >10 after a 30-minute incubation. This homogeneous assay can be used to continuously monitor product formation. Validated with known inhibitors, the IC50 value for one of the inhibitors determined with SensoLyte™ 520 beta-secretase assay kit was consistent with published data.

About AnaSpec, Inc.

AnaSpec, Inc. is a leading provider of integrated proteomics solutions to pharmaceutical, biotech, and academic research institutions throughout the world. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and combinatorial chemistry. Established in 1993, AnaSpec's ISO9001:2000 certified headquarters and manufacturing facilities are located in San Jose, CA.

References:

1. Selkoe DJ. Nature 399: A23 (1999)
2. Suzuki, N., et al. Science 264, 1336 (1994)
3. Iwatsubo, T. et al. Neuron 13, 45 (1994)
4. Qi-Takahara, Y. et al. J. Neurosci. 25, 436 (2005)
5. Zhao, G. et al. J. Biol. Chem. 280, 37689 (2005)
6. Mullan, M. et al. Nat.Genet. 1, 345 (1992)

 
 
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AnaSpec Introduces New SensoLyte™ Beta-Secretase Assay Kit

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Contact: Ping Yang 
408-452-5055 ping[.]anaspec.com
 
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IMPORTANT INFORMATION: Issuance, publication or distribution of this press release in certain jurisdictions could be subject to restrictions. The recipient of this press release is responsible for using this press release and the information herein in accordance with the applicable rules and regulations in the particular jurisdiction. This press release does not constitute an offer or an offering to acquire or subscribe for any AnaSpec, Inc. securities in any jurisdiction including any other companies listed or named in this release.

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