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AnaSpec Announces the Release of A Product Line of Recombinant Protein A, Conjugated with HiLyte™ Fluor Dyes or Biotin - AnaSpec is pleased to announce the release of a product line of recombinant Protein A, conjugated with HiLyte™ Fluor dyes or Biotin. This universal reagent can be used to detect antibodies from different species, such as human, rabbit, mouse, etc
AnaSpec Announces the Release of A Product Line of Recombinant Protein A, Conjugated with HiLyte™ Fluor Dyes or Biotin

 

PRZOOM - /newswire/ - Fremont, CA, United States, 2015/01/28 - AnaSpec is pleased to announce the release of a product line of recombinant Protein A, conjugated with HiLyte™ Fluor dyes or Biotin. This universal reagent can be used to detect antibodies from different species, such as human, rabbit, mouse, etc.

   
 
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AnaSpec is pleased to announce the release of a product line of recombinant Protein A, conjugated with HiLyte™ Fluor dyes or Biotin. This universal reagent can be used to detect antibodies from different species, such as human, rabbit, mouse, rat and others. Protein A is available as labeled with HiLyte™ Fluor 488 (Ex/Em = 499/523 nm), HiLyte™ Fluor 555 (Ex/Em = 553/568 nm), HiLyte™ Fluor 647 (Ex/Em = 649/674 nm), HiLyte™ Fluor 680 (Ex/Em = 678/699 nm), and HiLyte™ Fluor 750 (Ex/Em = 754/778 nm), or biotin. The large panel of emission wavelength HiLyte™ labeled Protein A enables the visualization of different target proteins within one species. Additionally, HiLyte™ Fluor dyes exhibit brighter signal compared to classic dyes (FITC, FAM, TAMRA, etc.).

Protein A is a non-glycosylated cell wall protein of Staphylococcus aureus that can bind the Fc part of immunoglobulin molecule of different species with strong affinity (1-4). Protein A consists of five IgG binding domains (A, B, C, D, and E), each approximately 60 amino acids long with no cysteines present and two S. aureus cell membrane binding domains - X and M (3).

AnaSpec’s recombinant Protein A consists of only IgG binding domains and was expressed in E.coli. Its apparent molecular weight is approximately 30,000 Da compared to the most reported 42,000 Da for the native Protein A that is found in S. aureus.

Recombinant Protein A conjugated with HiLyte™ Fluor 488 can be used for immunoglobulin detection in IHC and IF and for assay development. Biotinylated Protein A can be used in the aforementioned applications, and for western blotting.

References
1. Björk, I. Eur J Biochem 29 (1972):579-584.
2. Sjödahl, J. Eur J Biochem 78 (1977): 471-490.
3. Schröder, A. K. et al. Immunol 57 (1985): 305-309.
4. Rosenberg, I.M. Protein Analysis and Purification, Birkhäuser, 2005: 41-47.

About AnaSpec, EGT Group
AnaSpec (anaspec.com), EGT Group is a leading provider of integrated proteomics and genomics solutions™ for worldwide life science research. AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, amino acids, oligonucleotides, and Takyon™ qPCR MasterMixes. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening (HTS) and drug discovery. AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development, fluorescent dyes, and oligonucleotide synthesis. AnaSpec is certified to ISO 9001:2008.

For more information visit: AnaSpec's website.

 
 
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AnaSpec Announces the Release of A Product Line of Recombinant Protein A, Conjugated with HiLyte™ Fluor Dyes or Biotin

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Contact: Cecilia Po - AnaSpec.com 
510-791-9560 cecilia[.]anaspec.com
 
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IMPORTANT INFORMATION: Issuance, publication or distribution of this press release in certain jurisdictions could be subject to restrictions. The recipient of this press release is responsible for using this press release and the information herein in accordance with the applicable rules and regulations in the particular jurisdiction. This press release does not constitute an offer or an offering to acquire or subscribe for any AnaSpec, Inc. securities in any jurisdiction including any other companies listed or named in this release.

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