The 2006 Frost & Sullivan Product Innovation of the Year Award in gene expression analysis technologies is presented to Miltenyi Biotec GmbH for its creation of a unique PrepProtect™ Stabilization Buffer. PrepProtect Buffer is non-toxic and stabilises the RNA, DNA or protein in cell or tissue samples for relatively long periods. It thereby prevents RNA degradation before isolation and analysis, and ensures more reliable downstream processing.
The parallel handling of multiple biological samples and quick processing are critical for accurate downstream analysis such as gene expression profiling. While this process requires high-quality RNA, chemical instability and susceptibility to ubiquitous RNases create major challenges. In this context, the PrepProtect Buffer enables much-needed RNA stabilisation.
"PrepProtect allows RNA stabilisation, in both freshly prepared as well as previously frozen samples," notes Frost & Sullivan Research Analyst Sirisha R S. "A fresh cell or tissue sample mixed with PrepProtect Solution can be stabilised for at least one day at 37 degreesC, one week at 18 degreesC to 25 degreesC, one month at 4 degreesC or for archival storage below -20 degreesC."
The PrepProtect Solution prevents the degradation of RNA when direct analysis or quick-freezing, post sample collection, is not possible and when samples are being transported without dry ice or frozen tissue needs to be weighed and dissected. Due to its ability to facilitate sample collection and the parallel processing of multiple samples, as well as to allow samples to be shipped without the use of dry ice, the PrepProtect Solution has emerged as a reliable tool for downstream applications.
"PrepProtect also has the ability to seep through previously frozen material, and protects RNA during thawing," adds Ms. Sirisha. "When the frozen samples are incubated in the PrepProtect solution, RNA in cells are stable for up to one hour at room temperature or overnight at 4 degreesC, thereby helping researchers prepare samples and weigh or dissect tissues before RNA isolation."
PrepProtect Buffer also reduces the hardness of the frozen tissues by softening their structure. It thus facilitates tissue lysis and homogenisation without prior pulverisation with mortar and pestle. Furthermore, it helps to preserve both the quantity and quality of RNA for downstream analysis such as quantitative polymerase chain reaction (PCR) or microarray analysis. Moreover, along with the RNA stabilisation, PrepProtect Buffer preserves the integrity of DNA and denatured proteins in biological samples.
"Miltenyi Biotec has successfully developed a wide product portfolio with its MACS® Technology-based solutions for basic biomedical and clinical research, particularly for cell separation, cell analysis, cell culture, molecular biology, and clinical research applications," states Ms. Sirisha. "At least three or four products are released in the market every month, and this exemplifies the productivity of the company's R&D initiatives and its commitment to product innovation."
The Frost & Sullivan Product Innovation Award is presented each year to the company that has demonstrated excellence in new products and technologies within their industry. The recipient company has shown innovation by launching a broad line of emerging products and technologies.
Frost & Sullivan Best Practices Awards recognise companies in a variety of regional and global markets for demonstrating outstanding achievement and superior performance in areas such as leadership, technological innovation, customer service, and strategic product development. Industry analysts compare market participants and measure performance through in-depth interviews, analysis, and extensive secondary research in order to identify best practices in the industry.
About Frost & Sullivan
Frost & Sullivan, a global growth consulting company, has been partnering with clients to support the development of innovative strategies for more than 40 years. The company's industry expertise integrates growth consulting, growth partnership services and corporate management training to identify and develop opportunities. Frost & Sullivan serves an extensive clientele that includes Global 1000 companies, emerging companies, and the investment community, by providing comprehensive industry coverage that reflects a unique global perspective and combines ongoing analysis of markets, technologies, econometrics, and demographics.
About Miltenyi Biotec
Miltenyi Biotec, founded in 1989, has become a successful diversified biotechnology company. With subsidiaries and distributors around the world, the company has almost 1000 employees.
The product portfolio comprises more than 800 products for cell separation, cell analysis, cell culture, and molecular biology in basic and biomedical research.
Miltenyi Biotec invented and developed MACS® Technology, the gold standard method in magnetic cell separation. MACS® Technology offers a multitude of applications: from small-scale to large-scale cell purifications, from cell to biomolecule separations, from lab bench to clinical research applications.
With the MACSmolecular product range, Miltenyi Biotec offers innovative reagents, instruments, and services for molecular cell analysis. This includes highly specific molecule purifications and revolutionary in-column enzymatic reactions with convenient MACS® Technology, single-cell analysis, as well as cutting-edge gene expression profiling products and services.
For further information, please contact:
Prof. Dr. Uwe A. O. Heinlein
Head of Corporate and Marketing Communications
Miltenyi Biotec GmbH