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AnaSpec Introduces SensoLyte« ThT A▀42 & A▀40 Aggregation Kits - SensoLyte« ThT A▀40 and A▀42 Aggregation kits provide a convenient method to measure A▀40 and A▀42 aggregation
AnaSpec Introduces SensoLyte« ThT A▀42 & A▀40 Aggregation Kits

 

PRZOOM - /newswire/ - Fremont, CA, United States, 2012/11/06 - SensoLyte« ThT A▀40 and A▀42 Aggregation kits provide a convenient method to measure A▀40 and A▀42 aggregation.

   
 
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SensoLyte« ThT A▀40 and A▀42 Aggregation kits, using the fluorescent dye Thioflavin T (ThT), provide a convenient and standard method to measure A▀40 and A▀42 aggregation, respectively. In each of the kits, A▀40 or A▀42 peptide is pretreated to ensure it is in a monomeric state. Included in the kits is an optimized fibrillation buffer and two known inhibitors as controls. The assay is based on the property of ThT dye in which fluorescence (Ex/Em=440/484 nm) increase is proportional to the amount of dye bound to aggregated A▀ peptides.

Alzheimer's disease (AD), the most common cause of dementia, is characterized by the presence of senile plaques and neurofibrillary tangles, surrounded by damaged neurons. Beta-Amyloid (A▀) peptides, A▀40 (1-40) and A▀42 (1-42), were found to be a major component of the above plaques. Many studies suggest that these peptides can form toxic oligomers and fibrils under physiological conditions and rapidly aggregate. Since A▀ aggregation is evidently an essential event in the pathogenesis of AD, a reliable assay is important to study A▀ fibrillation kinetics and for screening A▀ aggregation inhibitors.

References
1. Hellstrand E., et. al., ACS Chem. Neurosci., (2010) 1: 13-18.
2. Karafin A., et. al., MEDIMOND, (2009) 11-15: 255-259.
3. Hudson S. A., et. al., FEBS J., (2009) 276: 5960-5972.
4. Liu R., et. al., Neurobiol. of Disease, (2005) 20: 74-81.
5. Kudva Y. C., et. al., Biochem. J., (1998) 331: 809-813.

 
 
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AnaSpec Introduces SensoLyte« ThT A▀42 & A▀40 Aggregation Kits

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Contact: Tiffany Kao - AnaSpec.com 
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